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- Clean around and inside the incubation house using a broom. Always inspect for cleanliness before entering with new spawn bags.
- After inoculation, transfer substrate bags to mushroom incubation house.
- Place bags on shelves for incubation.
Bags can be placed horizontally or vertically, which takes more space.
Note: At the beginning, little ventilation and light should be allowed. After about 10 days, there ventilation should regulate the desired temperature. After 20–25 days, area should be well ventilated and more light can be let in for constant monitoring.
- Visually check mycelium on a daily basis looking for abnormal mycelium (such as black spots, green spots, brown spots, orange or red spots).
Try to find out the causes of abnormal mycelium (PEST, DISEASE).
- Move bags to fruiting body area (or prepare for fruiting).
- Separate contaminated bags and pasteurize again; or separate partially contaminated bags and open them; or tear the plastic bags off and reuse sawdust; or ferment substrate as compost for gardening.
(See waste management).
- Observe and collect data.
Take notes before drawing conclusions.