Growing Tips

Tissue Cultures

  1. Prepare materials:

Potatoes: 200 gr.
Dextrose: 20 gr.
Agar powder: 20 gr.
Water: 1 liter.
Cotton (gauze)

Note: Visually check potatoes for spots or rot. Buy dextrose and Agar of commercial grade.

  1. Wash and cut potatoes into one-centimeter cubes; leave on or remove the skin.
  2. Clean small flat bottles (small whiskey bottles as a container can be used).
  3. Place potatoes in one liter of water. Simmer for 15 – 20 minutes.
  4. Remove potatoes & keep the broth as clear as possible.

Add water to broth to reach one liter of liquid PDA

  1. Bring water to stove. Add dextrose followed by agar. Slowly stir continuously with regular speed until completely dissolved.
  2. Pour liquid PDA in bottle until you reach 5 – 10 mm high
  3. Plug bottle with cotton.
  4. Place bottles in autoclave at 121oC for 20 – 30 minutes to ensure complete sterilization.

Let cool down to around 37oC

  1. Place bottles in slanted position as to increase surface area of the medium. PDA should come close to the neck but must not touch the cotton plug.

After PDA medium is settled in bottle, transfer all bottles to clean shelf in the clean room.

  1. Check for contamination (contamination can be seen when dark spots or lines occur).

 

 

Selecting tissue culture

Do not want to use your own cultures to start here is a video for one of our YouTube friends who also sell cultures too.

 

  1. Prepare materials:
  • Special needle (insulated handle)
  • Alcohol lamp
  • Alcohol
  • Cotton (gauze)
  • Matches or lighter
  • Bottles with PDA
  • Laminar flow cabinet (or protected environment)
  • UV lamp
  1. Select a strong mushroom for culture.
  • Healthy.
  • Not too mature, not too young.
  • Not too humid (at least 2-3 hours after watering)
  • With a stiff stalk
  • Make sure it is clean and far from any contaminated mushroom.
  1. Clean the room, all necessary tools, inside and outside the laminar flow cabinet with alcohol. Transfer PDA bottles and necessary tools into the chamber.
  2. Place all cleaned materials inside laminar flow. Turn on UV lamp and laminar flow. After 10-15 minutes, turn off UV lamp but leave laminar flow for the duration of the operation.
  3. Clean both hands and bottles with alcohol and insert hands into the cabinet.
  4. Hold needle with 2 fingers in a 45o-degreeangle, flame needle to disinfect until the needle turns red. Make sure it does not touch any surface after flaming.
  5. While needle cools down (15-20 seconds – hold needle not to touch anything or place it on the clean surface of a glass).
  6. Using other fingers, tear mushroom lengthwise (DO NOT use knife to cut).
  7. With the needle, cut a small piece (2 mm x 2 mm) of fleshy tissue from inside the mushroom (in the middle between the cap and the stalk). Make sure that it is clean and did not touch the outside of the mushroom.
  8. Flame around the mouth of the bottle. Using other fingers, remove cotton plug of PDA bottle in front of flame to secure against contamination.
  9. Insert the needle in the bottle and inoculate by placing small piece of cut mushroom in the middle of the PDA’s surface. Make sure the piece of mushroom does not touch anything before entering the PDA bottle
  10. Close bottle immediately near the flame with cotton plug

Note: the bottom of the bottle should always be lower than the mouth of the bottle and the mouth of the bottle should remain near the flame at all times.

  1. Label bottles and indicate: Date, type of mushroom, mother spawn #.

Mushroom Supplements

Something exciting is happening with Mushrooms!   What is amazing is that consumers are catching on about how healthy mushrooms are. Mushroom supplements are the newest sign of this amazing trend. Several growers are realizing that it can be very profitable to...

Lab Tools for Mushroom Growers

Sometimes it is hard to find lab supplies when you are small and just starting out. Also, locating a place to purchase all of them at one time can be near impossible. Well, Amazon has much of what you may want or need if you know where to look. This page will just...

New HEPA Filter Laminar Flow Hood

This is a new model of flow hood now available on Amazon. It is too early to have a great deal of feedback but it is nice to see more options entering the market. As you know a Laminar flow hood is the next step for someone's mushroom growing operation often after a...

Mushroom Broth replaces bouillon

So many exciting products are being launched every week now in the mushroom business that we are watching these products for you. As a farmer or grower trying these products and seeing if they can fit in your own product line can mean more profit for your business....

Mushroom Nightlight

This bedside nightlight looks cool!   It seems like this nightlight would be a great way to get your kids interested in and loving mushrooms at an early age. Maybe a key to get them thinking positively about mushrooms and helping more on the farm? Made of...

Have You Tried Mushroom Coffee?

Coffee Drinker? Think you have tried it all before? How about Mushroom Coffee? Yes, mushroom coffee is now available and it is getting some solid reviews on Amazon and other marketplaces. Here are the details on this coffee. POWER ON CREATIVE FOCUS: Half the caffeine,...

Mushroom Video on Prime

Super Fungi - On Prime Video Mushrooms could be man's best allies in the struggle against sanitary and environmental challenges that threaten our societies. A select group of species, known as Super Fungi, are incredibly effective at tasks ranging from digesting oil...

Please follow and like us:
Social media & sharing icons powered by UltimatelySocial
YouTube
LinkedIn
Share