INOCULATING BAGS WITH SORGHUM SEEDS
Note: The inoculation room must be kept very clean and free of diseases to avoid contamination. Avoid sunshine from entering the area.
- Prepare materials:
- Alcohol lamp
- Sorghum coated with spawn
- Fire
- Square paper (5’’ x 5’’)
- Rubber bands
- Cotton Alcohol
- Pasteurized substrate bags
- Cleanroom with broom or dust vacuum Rub the floor with a disinfecting solution.
- Bring substrate bags from pasteurization chamber to the inoculation area.
- Light alcohol lamp.
- Disinfect hands and pasteurized substrate bags with alcohol.
- Take a bottle of sorghum coated with spawn (all-white color).
- Shake well bottle against car tire (or old tire) to release sorghum seeds and then, bring bottles to inoculation room. Do not open bottle
- A flame around the bottleneck. Open bottle very close to the flame to take out the air from inside.
- Drop 10 – 20 sorghum seeds coated with spawn in the mushroom bag. Act very quickly and with very little movement.
- Then bring cotton to close the bag as quickly as possible. Repeat for all bags.
- Leave bottle near the flame until all bags have been inoculated. Then close the bottle.
- Following inoculation of all bags, place clean square paper to cover the top of the bag and tie with a rubber band.
- Bring bags to incubation room.
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